WebSep 1, 2015 · The proteins produced by the baculovirus transfected insect cells as well as the S2 insect cells were designed to be secreted into the culture supernatant during expression. Such proteins have been processed through ER and Golgi and contain putative glycosylations and disulfide bonds. The recombinant proteins were purified from the … WebType Cell Line Cat. No. Size SFM Adapted Cat. No. Size Insect D. Mel 2 10831-014 3 ml Drosophila-SFM 10797-017 500 ml 10797-025 1,000 ml Insect High Five ™ Cells 3855-02 3. x. 10 6 cells/ml Express Five ® SFM 10486-025 1,000 ml Insect Sf9 11496-015 3 ml Sf-900 II SFM 10902-096 500 ml 10902-088 1,000 ml 10902-070 10 L
Protein Expression in the Drosophila Schneider 2 Cell System
WebAug 31, 2011 · To test whether more than two proteins can be processed from a multicistronic vector in insect cells, we generated the vector pAc5-STABLE2-Neo. Again, we used the Thosea asigna T2A peptide to... WebApr 6, 2005 · In this respect, recombinant β-secretase has been expressed in insect cells using baculovirus-insect cell systems (Charlwood et al., 2001, Bruinzeel et al., 2002) and Drosophila melanogaster Schneider 2 (S2) expression systems (Mallender et al., 2001). A high mannose glycosylated β-secretase reduced enzyme activity up to 50% in baculovirus ... magnolia on gaston dallas
Process Optimization for Recombinant Protein Expression in …
WebFeb 1, 2007 · The recombinant S2 cells were generated either by the transfection with a single plasmid containing an expression cassette and a selection cassette or by co-transfection with two separate... WebThe factors that most affect the growth and gene expression of S2 cells, namely cell line, cell passage, inoculum concentration, culture medium, temperature, dissolved oxygen … WebThe factors that most affect the growth and gene expression of S2 cells, namely cell line, cell passage, inoculum concentration, culture medium, temperature, dissolved oxygen concentration, pH, hydrodynamic forces and toxic metabolites, are discussed by comparison with other insect and mammalian cells. magnolia one superyacht