Fluorochrome spectra
WebThe excitation spectrum of a given fluorochrome is determined in a similar manner by monitoring fluorescence emission at the wavelength of maximum intensity while the fluorophore is excited through a group of consecutive … WebFor fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm.
Fluorochrome spectra
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WebApr 14, 2024 · Excitation spectrum When a fluorochrome is exposed to light, a photon with the appropriate amount of energy can excite an electron in the fluorochrome. In this … WebPE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.
Web164 rows · This is a table of some characteristics of fluorochromes useful for flow … WebPerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which …
WebCyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm. WebThe BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine …
WebBrightness of various fluorochrome conjugates Freshly isolated lymphocytes, stained with anti-human CD4 (RPA-T4) conjugated with various fluorochromes run on a BD LSR II flow cytometer. The fluorochromes were ranked based on observed stain index values. This chart is meant as a guideline of relative stain indices of various fluorochromes.
WebFluorochrome Guides Cytek’s fluorochrome guides provide examples of many dyes full spectrum signatures along with stain indices, spread information, and other useful … how many cups of rice in a kilogramWebFluorescence SpectraViewer Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. high schools manhattanWebBD Horizon™ Red 718 (R718) is an innovative small-molecule fluorochrome developed exclusively for BD Biosciences. It provides improved sensitivity and resolution of low … how many cups of shortening in 1 lbWebThe BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine appropriate filters to detect a fluorochrome as well as fluorochrome compatibility … high schools manhattan ksWebThe fluorochrome is excited by the blue laser (488 nm) and its emission peak is at 514 nm. Improved brightness in combination with 25% less spillover into the PE channel enables optimal detection of rare surface … how many cups of salt in 1kgWebFluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1,000 dyes … high schools massachusettsWebThis is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. how many cups of rice to feed 30 people