Binary vector pcambia-1300

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August undefined, 2024

WebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields http://www.biovector.net/product/1319.html

Two type III effector genes of Xanthomonas oryzae pv. oryzae …

WebJun 1, 2007 · The cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants. WebEnter the email address you signed up with and we'll email you a reset link. ghf59

Production of marker-free and RSV-resistant transgenic rice using …

WebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β … WebNov 9, 2015 · For genetic complementation, the full-length genomic DNA of the VPT1 fragment was amplified from wild type genomic DNA and cloned into the binary vector pCAMBIA-1300. To generate the VPT1VPT1 coding sequence without a stop codon, and GFP (35S:VPT1-GFP) were cloned into the pCAMBIA-1300. The tonoplast marker, … WebDec 18, 2008 · Vector Construction A transformation vector was constructed and denoted pCHO. It was derived from the Agrobacterium tumefaciens binary vector pCAMBIA 1300 ( http://www.cambia.org.au) by replacement of the original hph cassette driven by the 35S promoter from CaMV with newly constructed cassettes. chris yesawich

pCambia1300 Plant Expression Vector (ab275754) Abcam

WebNov 15, 2024 · The pCambia-1300 binary vector was used as a backbone. A pCambia–BSCTV vector was used as template to amplify pV86 or pC86 trans-activated promoters using primers pV86-GUS-R1-F/R or pC86 … Web作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。. 通过Google搜索"Biovector+您所需资源名称",您一定能找到所需的资源和技术服务。. 如有需要请联系：. Dr Xu,Senior Scientist. 电邮 ... chris yesson zslWebBinary vector pCAMBIA-1300, complete sequence Binary vector pCAMBIA-1300, complete sequence. Your browsing activity is empty. Activity recording is turned off. Turn … chris yetter

"WebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure … " - Binary vector pcambia-1300

Binary vector pcambia-1300

pCambia1301 Plant Expression Vector (ab275753) Abcam

WebJul 24, 2013 · A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase ( hpt) marker gene. WebPcambia 1300 (Search Vector Database) Vector type Plant Expression Selectable markers Hygromycin Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name FLAG-Pup (E) Promoter 35S Tag / Fusion Protein Triple Flag Tag …

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WebJul 1, 2024 · The pCAMBIA 1300 plasmid containing a GFP reporter gene driven by the Super promoter and harboring a Hygromycin resistance gene was used for transformation of somatic embryos. ... we transfected embryogenic calli with A. tumefaciens strain GV3101 containing a pCAMBIA 1300 binary vector, ... WebApr 7, 2024 · How to order pCAMBIA 1300 vector? Question. 3 answers. Apr 3, 2024; ... The steps include cloning your gene into a binary vector, transferred the vector into Agrobacterium, ...

WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a …

WebProduct name pCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for … WebClassification and research data for Binary vector pCAMBIA-1300, unclassified. Classification and research data for Binary vector pCAMBIA-1300, unclassified. Skip to …

WebJun 1, 2024 · The new plant RNAi binary vector, named pCAMBIA2301-GW-RNAi, has two inverted repeated Gateway cassettes driven by the cauliflower mosaic virus 35S (CaMV … ghf57.cnWebJun 19, 2007 · Biological Sciences Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of rice Akiko Sugio, Bing Yang, Tong Zhu, and Frank F. White Authors Info & … chris yetter 3gWebpCAMBIA2301 Agrobacterium binary vector for plant transformation, with kanamycin-resistance and GUS genes. Sequence Author: Cambia Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Plant Vectors More Plasmid Sets Home Plasmids Plant VectorspCAMBIA2301 Show … ghf59銆俢omWebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of … ghf59comWebThe pCambia1301 vector offers: High copy number in E.coli for high DNA yields. pVS1 replicon for high stability in Agrobacterium. Small size. Restriction sites designed for … chris yewWebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... chrisye youtubeWebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,... ghf600